The course is twisty and undulating, with no extended climbs but with quite a few punchy bits, especially on the unpaved parts. There are more than 40 km of gravel roads across twelve sectors (all shared with the Men’s course). The race starts near the Stadium/Fortezza Medicea in Siena. The first undulating kilometers on tarmac lead to the 2.1 km-long gravel sector 1 at km 18, which is perfectly straight and slightly downhill. Sector 2 (5.8 km) is just a few kilometres away; this will be the first tricky bit of the race, with a short descent followed by a long climb featuring sections over 10%. Upon reaching Radi, the route takes in the third gravel sector (4.4 km), shortly followed by sector 4 – named “La Piana” – that will lead the riders to Ponte d’Arbia. Shortly after, the route reaches Monteroni d’Arbia, which marks the beginning of sector 5 of San Martino in Grania (9.5 km) in the middle of the Crete Senesi. It’s a long sector marked by a sequence of gentle undulations in the first part, and ending with a twisting climb that leads back to paved road. The sixth dirt road sector, Montaperti, is only 800 m in length, but it greets the riders with double-digit gradients. The route goes back on tarmac in Vico d’Arbia where the 30 km circuit will begin.
The demanding final kilometers approach the city of Siena along wide and straight roads, connected by sweeping curves, initially downhill, and further on slightly uphill. With 2 km to go to the finish, the route takes the Via Esterna di Fontebranda; here the gradient touches 9%. The stone pavement begins 900 m before the finish line, just past the Fontebranda Gate. The gradient is over 10% until 500 m from the finish, reaching its steepest gradient of 16% along Via Santa Caterina. A sharp right-hand turn leads to Via delle Terme, and then to Via Banchi di Sotto. Starting 300 m to the finish onwards, the road is a slight, continuous ascent. With 150 m to go, a right turn leads into Via Rinaldini. The route enters the Piazza del Campo just 70 m from the finish line. The final 30 m descend at a gradient of 7% to the finish line, which is on level road.